Recombinant Mouse TrkB (C-6His

Recombinant Mouse TrkB (C-6His)(C06R)

 

产品说明(Description)

Recombinant Mouse Neurotrophic Tyrosine Kinase Receptor Type 2 is produced by our Mammalian expression system and the target gene encoding Cys32-His429 is expressed with a 6His tag at the C-terminus.

Accession #: P15209

Known as: BDNF/NT-3 Growth Factors Receptor; GP145-TrkB; Trk-B; Neurotrophic Tyrosine Kinase Receptor Type 2; TrkB Tyrosine Kinase; Tropomyosin-Related Kinase B; NTRK2; TRKB

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:         Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin:  Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY