产品名称:蛋白酶S
英文名称:Protease S Aureus
产品货号:Y13044
产品规格:1mg
CAS:66676-43-5
Activity:≥500units/mg
活力定义:One Unit causes a change of 0.001 A280nm per minute at 37℃,pH 7.8 using casein as the substrate
产品描述:Protease S.aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids.In the presence of ammonium,the enzyme specificity is limited to glutamic sites.It has a molecular weight of 27,000 daltons and optimum pH"s of 4.0 and 7.8 with hemoglobin as the substrate.Protease S.aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-,Cl-,CH3COO- and NO3.Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol.,45,469,1976).
储存条件:2~8℃
来源:金黄色葡萄球菌,V8
外观:粉末
用途:Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.
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