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Human CD47 Protein (Fc Tag)

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  • ¥2159.15
  • Frdbio
  • WUHAN,CHINA
  • FrdH3507-202H
  • 2025年10月12日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      <- 20℃

    • 保质期

      >2 years

    • 英文名

      Friendbio Science & Technology Co.,Ltd.

    • 库存

      200

    • 供应商

      福因德科技(武汉)有限公司

    • 规格

      50μg

    分子别名(Synonym):IAP,MER6,OA3 种属(Species):Human 表达系统(Expressed Host):HEK293 Cells 制剂(Buffer):Lyophilized from sterile PBS, pH 7.4. 分子量(Molecule Mass):The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing conditions. 内毒素(Endotoxin):<1.0 EU per μg by the LAL method. 生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His (Cat:H2836-802H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml. 表达区间(Protein Construction):A DNA sequence encoding the human CD47 (NP_942088.1) (Ser2-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus. 存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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    图标文献和实验
    相关实验
    • CD47/CD47R分子

      CD47/CD47R 分子 CD47 常用单克隆抗体或代号: BRIC126,CIKM1 主要表达细胞: 广泛 [AS] 分子质量(kDa)和结构: gp47,N连接葡萄糖 功 能: 粘附分子相关信号分子 CD47R 常用单克隆抗体或代号:(为原CDw149)MEM―33 主要表达细胞:广泛[NL] CD47 Aka integrin associated protein (IAP

    • Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA

      of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion

    • 干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密

      CUT&Tag(Cleavage Under Targets and Tagmentation)是蛋白-DNA 互作的一大革新技术,它不需要使用甲醛交联以及免疫共沉淀,而是通过针对靶蛋白(如转录因子、染色质重塑蛋白)的抗体和 Protein A/G 的介导,使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头,经 PCR 扩增后即可形成用于高通量测序的文库(见图 1 )。CUT &Tag 技术具有细胞投入量低、信噪

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