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- Elabscience
- 湖北武汉
- E-AB-F0995C
- 2025年07月14日
- FCM
- Rat
- Mouse
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- 技术资料
- 亚型：
Rat IgG2b, κ
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
FITC
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F0995C
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Rat
- 应用范围：
FCM
- 抗体英文名：
FITC Anti-Mouse F4/80 Antibody[CI:A3-1]_货号:E-AB-F0995C
- 抗体名：
FITC Anti-Mouse F4/80 Antibody流式抗体[CI:A3-1]_货号:E-AB-F0995C
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥576.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥336.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥864.0 |
F4/80 抗体，FITC标记 F4/80 抗体[CI:A3-1]，FITC标记抗小鼠 F4/80 抗体[CI:A3-1]
产品名称：FITC Anti-Mouse F4/80 Antibody[CI:A3-1]
货号：E-AB-F0995C
| Background |
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
|
| Alternate Names |
Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480;
|
| Swissprot |
Q61549
|
| Gene ID |
13733
|
| Clone No |
CI:A3-1
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
FITC Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
 |
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
from their mothers ii. discard un-needed non-transgenic pups iii. house males and females separately f) when mice are 6 weeks old they may be mated (see 1. above) ES Cell-Mouse Chimera Breeding Based on our experience, we propose the following
Purification Of Anti Peptide Antibody
the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol
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