- ￥1700 - 4000
- GeneTex
- 美国
- GTX100462
- 2025年07月15日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human PI3 kinase p110 alpha. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100462
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
2.58 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PI3 kinase p110 alpha
- 抗体英文名：
PI3 kinase p110 alpha antibody
- 抗体名：
PI3 kinase p110 alpha 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
PI3 kinase p110 alpha antibody detects PI3 kinase p110 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% PFA at RT for 15 min.
Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3 kinase p110 alpha(GTX100462) antibody.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (50 μg of whole cell lysate)
A: C2C12
B: C2C12+AEA10mM
GTX100462 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献和实验Chen HY et al., Antioxidants (Basel) 2021 (PMID:33451157)
Lin JT et al., Journal of Functional Foods 2018 47()
Zhang J et al., Redox Biol 2022 (PMID:36126419)
的I型PI3K. 哺乳动物 细胞中Ι型PI3K又分为IA和IB两个亚型, 他们分别从酪氨酸激酶连接受体和G蛋白连接受体传递信号.IA 型PI3K是由催化亚单位p110和调节亚单位p85所组成的二聚体蛋白, 具有类脂激酶和蛋白激酶的双重活性.PI3K通过两种方式激活, 一种是与具有磷酸化酪氨酸残基的生长因子受体或连接蛋白相互作用, 引起二聚体构象改变而被激活; 另一种是通过Ras和p110直接结合导致PI3K的活化. PI3K激活的结果是在质膜上产生第二信使PIP3, PIP3与细胞内含有PH结构
存在于胞质中，等待适当的信号激活。P13K通过两种方式激活：一种是与具有磷酸化酪氨酸残基的生长因子受体或连接蛋白相互作用，引起二聚体构象改变而被激活。对RTK而言，这种信号来自于配体介导的激酶的活化，结果使位于细胞膜内表面的酪氨酸残基磷酸化。磷酸化的酪氨酸残基立即成为细胞内信号蛋白的结合位点，通过与P85的SH2结构域结合而把P85―P110复合物聚集到细胞膜上并使之活化。另一种是通过Ras和Pll0直接结合，导致P13K的活化。PI3K被激活后，在细胞膜上生成第二信使PIPa，PIPa与细胞内含有PH
tyrosine kinases (RTKs) and the concomitant assembly of receptor–PI3K complexes. These complexes localize at the membrane where the p110 subunit of PI3K catalyses the conversion of PtdIns(4,5)P2 (PIP2) to PtdIns(3,4,5)P3 (PIP3). PIP3 serves as a second
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