![Chk1 (phospho Ser345) antibody [C1C2], Internal](https://img1.dxycdn.com/2023/0711/255/2344116916935858761-14.jpg)
- ￥1700 - 4000
- GeneTex
- 美国
- GTX100065
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding phospho Ser345 of human Chk1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100065
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.66 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Chk1 (phospho Ser345)
- 抗体英文名：
Chk1 (phospho Ser345) antibody [C1C2], Internal
- 抗体名：
Chk1 (phospho Ser345) 抗体 [C1C2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Untreated (–) and treated (+) C2C12 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Chk1 (phospho Ser345) stained by Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% PFA at RT for 15 min.
Green: Chk1 (phospho Ser345) protein stained by Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Sample: 20 μg of HCT116 P53 whole cell lysate 10% SDS PAGE
Chk1-phopho-S345 antibody
GTX100065 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Chk1 (phospho Ser345) (GTX100065) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Subramanian GN et al., J Cell Biol 2020 (PMID:32328643)
Luo J et al., mBio 2020 (PMID:32071277)
Hung CC et al., Oncotarget 2015 (PMID:25686830)
Yang C et al., Oncol Lett 2022 (PMID:35317026)
Sakata R et al., Cell Rep 2021 (PMID:33909997)
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