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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Primate
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX15456
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IP, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
No cross-reactivity is seen with presenilin 2. This antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1.
- 抗体英文名:
Presenilin 1 antibody [APS 11]
- 抗体名:
Presenilin 1 抗体 [APS 11]
- 规格:
200 μg
ICC/IF analysis of mouse fibroblasts using GTX15456 Presenilin 1 antibody [APS 11].
IHC-P analysis of human brain tissue using GTX15456 Presenilin 1 antibody [APS 11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
IHC-P analysis of human liver tissue using GTX15456 Presenilin 1 antibody [APS 11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
WB analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3) using GTX15456 Presenilin 1 antibody [APS 11].
Dilution : 1:200
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文献和实验11.33 ml 11.33 ml Gel A/B 5.37 ml 6.90 ml 8.44 ml 9.20 ml 9.97 ml 11.50 ml Gel A 583 µl 750 µl 916 µl 999 µl 1.08 ml 1.25 ml TEMED 14.84 µl 13.6 µl 12.36 µl 11.75 µl 11.13 µl 9.89 µl 10% APS 148.4 µl 136 µl 123.6 µl 117.5 µl 111.3 µl 98.9 µl
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, and has characterized pathways for the trafficking of APC both into and out of the nucleus.Antibody specificity is an important factor in the determination of APC localization, and in this chapterwe outline a strategy for the unambiguous detection of APC using
, lower (10%)(~30 mls for 2 gels: Acrylamide (40%) dH2 O 1M Tris (pH 8.7) 10% SDS 10% APS (Ammonium persulfate) TEMED 7.5ml 11.0ml 11.2ml 300µL 120µL 20µL Mix
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