- ￥2800
- GeneTex
- 美国
- GTX43100
- 2025年07月15日
- FACS
- Mouse
- Human, Cynomolgus monkey, Rhesus Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human endothelial cell membrane vesicles.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Human, Cynomolgus monkey, Rhesus Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX43100
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes the human CD34. Clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion.
- 抗体英文名：
CD34 antibody [QBEnd/10] (PE)
- 抗体名：
CD34 抗体 [QBEnd/10] (PE)
- 规格：
25 test
Figure A. A647 conjugated Mouse anti Human CD45 and RPE conjugated Mouse IgG1 isotype control (GTX76639). Figure B. A647 conjugated Mouse anti Human CD45 and RPE conjugated Mouse anti Human CD34 (GTX43100).All experiments performed on human peripheral blood lymphocytes.
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文献和实验Pilling D et al., J Leukoc Biol 2006 (PMID:16543402)
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
离心，收集界面层单个核细胞，与CD34-PE标记的单抗室温孵育15 min，待测。 1.3 FACS检测CD34+细胞 上述标记细胞悬液分为2管，其中一管6 h内使用流式细胞仪 (EPICS ELITE ESP， COULTER公司)测定CD34+细胞占单个核细胞(淋巴细胞和单核细胞)的百分率，另一管经1%多聚甲醛固定，4℃冰箱保存72 h后，FACS测定CD34+细胞数。 1.4 荧光标记的CD34单克隆抗体 33份APBSC同时分别用表达第2类抗原 表位QBEnd 10
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