- ￥5000
- GeneTex
- 美国
- GTX12178
- 2025年07月11日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is highly conserved (single amino acid substitution) in Tetrahymena histone H3.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX12178
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
2-2.5 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K9ac (Acetyl Lys9)
- 抗体英文名：
Histone H3K9ac (Acetyl Lys9) antibody
- 抗体名：
Histone H3K9ac (Acetyl Lys9) 抗体
- 规格：
50 μl
IHC-P analysis of mouse testis tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
IHC-P analysis of rat lung tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
IHC-P analysis of mouse liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
WB analysis of NIH3T3 cells using GTX12178 Histone H3K9ac (acetyl Lys9) antibody.
Lane 1 : Anti Histone H3K9ac (acetyl Lys9) antibody
Lane 2 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 (acetylated Lys9) peptide
Lane 3 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 peptide(non-acetylated)
IHC-P analysis of rat liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
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文献和实验Ishiguro K et al., J Immunol 2011 (PMID:21278340)
Liang DY et al., Mol Pain 2014 (PMID:25217253)
Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potential deacetylase enzymes
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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