- ￥5400
- GeneTex
- 美国
- GTX88007
- 2025年07月12日
- WB, ICC/IF, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Histone H3K9ac (1-50).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX88007
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K9ac (Acetyl Lys9)
- 抗体英文名：
Histone H3K9ac (Acetyl Lys9) antibody
- 抗体名：
Histone H3K9ac (Acetyl Lys9) 抗体
- 规格：
100 μg
ICC/IF analysis of HeLa cells using GTX88007 Histone H3K9ac (Acetyl Lys9) antibody. The picture on the right is blocked with the synthesized peptide.
WB analysis of Raw264.7 cells treated with TSA 400nM (24h) lysate using GTX88007 Histone H3K9ac (Acetyl Lys9) antibody. The lane on the right is blocked with the synthesized peptide.
IHC-P analysis of human lung carcinoma tissue using GTX88007 Histone H3K9ac (Acetyl Lys9) antibody. The picture on the right is blocked with the synthesized peptide.
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文献和实验Yen YT et al., Nat Commun 2021 (PMID:34911954)
Schiaffino L et al., Sci Rep 2018 (PMID:30150770)
Bankole O et al., Int J Mol Sci 2022 (PMID:35162978)
Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potential deacetylase enzymes
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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