- ￥5300
- GeneTex
- 美国
- GTX48811
- 2025年07月07日
- WB, ELISA
- Rabbit
- Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Bovine
- 目录编号：
GTX48811
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
VEGFA
- 抗体英文名：
VEGFA antibody
- 抗体名：
VEGFA 抗体
- 规格：
50 μg
Western blot using GeneTex's Protein-A Purified anti-bovine VEGF antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa. Approximately 2 μg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA diluted 1:10. The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. Other detection systems will yield similar results.
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文献和实验Cui Q et al., Cells 2022 (PMID:36078162)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
9.12 Cell Res：VEGFA 信号通路在血管稳态调控过程中的两面性
① Cell Res：VEGFA 信号通路在血管稳态调控过程中的两面性内皮细胞响应信号刺激对血管形态、稳态和功能的调控至关重要。血管内皮生长因子 A (VEGFA) 自 20 世纪 80 年代被鉴定以来，其主要功能之一便是促进血管生长。中国科学院上海营养与健康研究所潘巍峻研究组在斑马鱼中发现，在 VEGFA 刺激条件下，cds2 基因突变胚胎的血管能够发生血管退化。斑马鱼活体成像技术表明，cds2 突变体的内皮细胞受 VEGFA 刺激能够通过逆向迁移和细胞凋亡来诱导血管退化。小鼠
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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