- ￥1700 - 4000
- GeneTex
- 美国
- GTX100817
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC
- Rabbit
- Human, Mouse, Sheep
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Sheep
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100817
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC
- 浓度：
1.76 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Alkaline Phosphatase (Tissue Non-Specific)
- 抗体英文名：
Alkaline Phosphatase (Tissue Non-Specific) antibody
- 抗体名：
Alkaline Phosphatase (Tissue Non-Specific) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (GTX100817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using alkaline phosphatase (liver/bone/kidney)(GTX100817) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Boos AM et al., Int J Nanomedicine 2014 (PMID:25429218)
Greenbaum AM et al., Blood 2012 (PMID:22323481)
Manal Matoug-Elwerfelli et al., Sci Rep 2020 (PMID:33299073)
Kengelbach-Weigand A et al., NPJ Regen Med 2021 (PMID:34413320)
Yen Y.T. et al., Commun Biol. 2021 (PMID:34079065)
Ou L et al., Ecotoxicol Environ Saf 2021 (PMID:33396178)
Agustina H et al., Patholog Res Int 2018 (PMID:29854380)
Chen YW et al., Materials Science and Engineering: C 2018 91()
Agustina H et al., Hindawi 2018 (Epub)
Wang JY et al., J Ethnopharmacol 2017 (PMID:28040510)
Shie MY et al., Materials Science and Engineering: C 2017 73()
Weigand A et al., Tissue Eng Part A 2016 (PMID:27998239)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
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