- ￥5000
- GeneTex
- 美国
- GTX32224
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of IKB alpha. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX32224
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
IKB alpha (phospho Ser32/Ser36)
- 抗体英文名：
IKB alpha (phospho Ser32/Ser36) antibody
- 抗体名：
IKB alpha (phospho Ser32/Ser36) 抗体
- 规格：
100 μl
WB analysis of TNFa-treated HepG2 (A), TNFa-treated Raw264.7 (B), TNFa-treated PC12 (C) whole cell lysates using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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文献和实验Gao F et al., Adv Sci (Weinh) 2019 (PMID:30989021)
Hu Y et al., Mol Ther Nucleic Acids 2020 (PMID:32160704)
【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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