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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 克隆性:
多克隆
- 浓度:
2mg/1ml
- 抗体英文名:
Goat Anti-rabbit IgG
- 规格:
1mg
| 英文名称 | Goat Anti-rabbit IgG |
| 中文名称 | 羊抗兔IgG |
| 别 名 | Immunoglobulin G H&L. |
| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rabbit, Quail |
| 产品应用 | not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 150kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 10mg/1ml |
| 免 疫 原 | Full length plasma protein:full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M PBS(pH7.4) |
| 保存条件 | Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔IgG 定量 EIA 试剂盒使用说明 原理 本实验采用双抗体夹心 ELISA 法。用抗兔 IgG 单抗包被于酶标板上,标准品和样品中的 IgG 与单抗结合,加入酶标抗体,形成免疫复合物连接在板上,加入酶底物TMB ,出现蓝色,加终止液硫酸,颜色变黄,在 4 50 nm 处测 OD 值
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
产生。例如,特异性抗体和抗酶抗体均由兔产生,再用羊抗兔IgG作为桥抗将两者连接起来。 PAP法的基本原理与酶桥法相似,均是利用桥抗将酶连接在第一抗体结合的部位,所不同的是,先将酶(过氧化物酶)和抗酶(过氧化物酶)抗体(PAP)制成复合物,以代替酶桥法中的抗酶抗体和随后结合的酶,将两步合并为一步。这一重要的改进,不仅简化了操作步骤,而且大大提高了敏感性。PAP法的不足之处是反应步骤多,需时长,在实际操作中,会带来非特异性放大效应。亲和免疫组织化学结合了免疫酶组织化学在待检抗原部位形成有色沉淀和亲和组织
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