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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 克隆性:
多克隆
- 浓度:
2mg/1ml
- 抗体英文名:
Rabbit anti-mouse Kappa light chain/FITC
- 规格:
100ul
| 英文名称 | Rabbit anti-mouse Kappa light chain/FITC |
| 中文名称 | FITC标记的兔抗小鼠k链 |
| 别 名 | Ig kappa chain C region; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; kappa light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809. |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | IgG |
| 产品应用 | Flow-Cyt=1:100-1000 IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 2mg/1ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Kappa li: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类,即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链(L)和两个重链(H)的单体组成。Ig 的轻链是相同的,有 κ(kappa)和 λ(Lambda)两种型别。五类 Ig 的重链结构不同,这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ(gamma)链和 μ(mu )链。 重链和轻链的 N 端的氨基酸排列顺序
到。 TUNEL检测: DNA链的断裂末端可以通过酶标记作用进行标记。在70%乙醇中固定之前,细胞在1%多聚甲醛中固定以将小的DNA片段结合到细胞中去,然后和Tdt以及一种已标记的核苷一起进行培育。所用的这些核苷包括:地高辛-dUTP,用经FITC标记的抗地高辛配基进行检测; 生物 素-dUTP,用经FITC标记的 抗生素 蛋白进行检测;溴-dUTP,用抗BrdUrd的FITC进行检测。 在加入PI标记DNA后,绿(FITC)与红(PI)相对的荧光能够进行记录。凋亡的细胞会发出绿色的荧
B淋巴细胞膜表面免疫球蛋白(SmIg)的检查 直接免疫荧光法
一、实验原理 SmIg是B细胞的抗原识别受体,也是B细胞特异的表面标志,用直接免疫荧光法可检查出SmIg。用荧光素标记的抗Ig抗体,在一定条件下与淋巴细胞混合,荧光素标记的抗Ig抗体可以与B细胞表面的Ig结合,在荧光显微镜下观察可见B细胞膜上出现荧光。此方法可用来鉴定B淋巴细胞。 二、实验材料 1. ICR小鼠(北京大学医学部实验动物中心提供) 2. 异硫氰酸荧光素(FITC)标记的兔抗小鼠Ig抗体(购自BD公司) 3. Hank's液:pH7.4(内含
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