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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 克隆性:
多克隆
- 浓度:
2mg/1ml
- 抗体英文名:
Donkey Anti-Guinea pig IgG/Alexa Fluor 647
- 规格:
100ul
| 英文名称 | Donkey Anti-Guinea pig IgG/Alexa Fluor 647 |
| 中文名称 | Alexa Fluor 647标记的驴抗豚鼠IgG |
| 别 名 | Immunoglobulin G |
| 抗体来源 | Donkey |
| 克隆类型 | Polyclonal |
| 交叉反应 | Gpig |
| 产品应用 | Flow-Cyt=1:100-1000 IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 150kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 2mg/1ml |
| 免 疫 原 | Full length plasma protein: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验保持优异的水溶性,为该波段最亮的橙色荧光染料。例如:同等的标记程度下,CF543 标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。CF 555、AlexaFluor 555、Tetramethylrhodamine(TAMRA) 等匹配Cy3滤光片的橙色荧光染料;红色(568-594nm处激发)CF 568、Alexa Fluor® 568,ATTO 565, Rhodamine Red等568nm处红色荧光染料;CF 568染料耐光性最好;高效水溶
')2 Fragment(Alexa Fluor® 555 Conjugate)#4413 Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠: Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408 Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor
Laboratories、Sigma、Bio-Rad 及DAKO公司。 对于免疫荧光检测实验(包括免疫细胞化学及免疫组织化学),应用最广泛的是耦联有Alexa Fluor系列染料的抗体(如Alexa Fluor 488, 546, 568, 594, 596, 647, 633)、花青染料(Cyanine)系列标记的抗体(如Cy2, Cy3, and Cy5)、FITC及罗丹明(Rhodamine)。其中对于Alexa Fluor标记的二抗,推荐选择Invitrogen 公司
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