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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 克隆性:
多克隆
- 浓度:
2mg/1ml
- 抗体英文名:
Donkey Anti-human IgG/Alexa Fluor 555
- 规格:
100ul
| 英文名称 | Donkey Anti-human IgG/Alexa Fluor 555 |
| 中文名称 | Alexa Fluor 555标记的驴抗人IgG |
| 抗体来源 | Donkey |
| 克隆类型 | Polyclonal |
| 交叉反应 | hu |
| 产品应用 | IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 160kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 2mg/1ml |
| 免 疫 原 | Full length plasma protein: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验感;良好的稳定性和活性染料的标记率。Alexa Fluor 488在较宽的PH值范围内保持稳定(PH4~10);FITC激发波长488nm,最大发射波长525nm;缺点荧光强度易受PH值影响,PH值降低时其荧光强度减弱。橙红色(543-555nm处激发) CF 543、AlexaFluor 546、ATTO550, Cy 3,DyLight 549, Rhodamine (TRITC) 匹配543nm的橙色荧光染料;CF ? 543 荧光条带明亮,耐光,水溶性好,确保了CF 543染料与抗体的耦联物
技术原理与方法|超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色(Alexa Fluor405,蓝色);CB1 染色(Alexa Fluor488,绿色);VGluT3 染色(Cy3,红色);DGLα染色(Alexa Fluor647,白色)。 大麻素合成酶 DGLα(白色)和大麻素受体 CB1(绿色)聚集在小鼠
')2 Fragment(Alexa Fluor® 555 Conjugate)#4413 Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠: Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408 Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor
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