Carbonic Anhydrase VIII/CA8 蛋白|Carbonic Anhydrase VIII/CA8 protein|Carbonic Anhydrase VIII/CA8(Mouse, His Tag)
- ￥4520
- Sino Biological
- 50767-M08E
- 2025年08月12日
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- 技术资料
- 规格：
100 µg
蛋白名称：Carbonic Anhydrase 8/CA8蛋白, Carbonic Anhydrase 8/CA8 protein
蛋白构建：A DNA sequence encoding the mouse CA8 (P28651) (Met 1-Gln 291) was expressed, with a C-terminal polyhistidine tag.
表达宿主：E. coli
蛋白纯度：> 88 % as determined by SDS-PAGE
蛋白活性：Measured by its esterase activity . The specific activity is >5 pmoles/min/μg.
蛋白内毒素：Please contact us for more information.
预测N端：Met 1
蛋白分子量：The recombinant mouse CA8 comprises 301 amino acids and has a calculated molecular mass of 34.5 kDa. The recombinant protein migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P28651
蛋白氨基酸序列：Met1-Gln291
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验碳酸酐酶 carbonic anhydrase carbonatehydrolyase
为催化 H2 CO3 CO2 ＋ H2 O反应的酶，是锌蛋白质（动物原性）， EC 4． 2． 1． 1，存在于脊椎动物的红血球和许多动物的各种组织以及植物的叶中。它在红血球中具有对碳酸和重碳酸离子的迅速转换的作用。在胃中对盐酸的分泌起作用，一般来说，具有调节体液 pH的作用。另外认为与植物的光合成有关系。
【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
News and Views: A prize catch for diabetic retinopathy Nature Medicine - 13, 131 - 132 (2007) Article: Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation Nature
Preparation and Characterization of Single-Enzyme Nanogels
shell. The effectiveness of this method is demonstrated with horseradish peroxidase (HRP), carbonic anhydrase, and lipase. The impacts of such an encapsulation on the activity and stability of enzymes are also discussed.
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