相关产品推荐更多 >

DLL1重组蛋白|Recombinant Human DLL1 / Delta-1 Protein (His Tag)
¥2310
ARAF重组蛋白|ARAF Protein
¥2380
Nogo Receptor / RTN4R 蛋白|Nogo Receptor / RTN4R protein|Nogo Receptor / RTN4R(Human, His Tag)
¥3870
重组人 Latexin / LXN / TCI 蛋白 (His标签)
¥3870
Neuroligin 3 / NLGN3 蛋白|Neuroligin 3 / NLGN3 protein|Neuroligin 3 / NLGN3(Human, His Tag)
¥4520
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 规格:
100 µg
蛋白名称:BID蛋白, BID protein
蛋白构建:A DNA sequence encoding the human BID isoform 1 (P55957-1) (Met 1-Asp 195) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL. 3. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant human BID consists of 197 amino acids and migrates as an approximately 22 kDa band in SDS-PAGE under reducing conditions as predicted.
蛋白NP号:P55957-1
蛋白氨基酸序列:Met1-Asp195
蛋白标签:N-cleavage
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
技术资料暂无技术资料 索取技术资料





