Recombinant human CD33 (AAH28152.1, Met1-His259) are conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Labeling Rate:
The FITC to protein molar ratio is 1-3
Formulation:
Aqueous solution containing 0.5% BSA and 0.03%Proclin 300
Concentration:
10 μl/Test, 0.1 mg/ml
Form & Shipping:
Liquid. Shipping at ambient temperature.
Storage:
This reagent is stable for 6 months from date of receipt when stored at 2℃ - 8℃. Protected from prolonged exposure to light. Do not freeze !
Usage:
The 5×10E5 cells are suspended in 50 μL washing buffer (1 X PBS + 1% BSA), add 10 μL CD33-FITC, incubate for 45 min at 4℃ from light, then wash 2 times with wash buffer. Analyze by flow cytometry immediately.