- ￥1740 - 5590
- Sino Biological
- 北京
- 11188-H86H-SD
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human B7-H3 Protein-AF488 Labeled (ECD, His Tag, Site-Specific)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥1740.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥5590.0 |
B7-H3重组蛋白|Recombinant Human B7-H3 Protein-AF488 Labeled (ECD, His Tag, Site-Specific)（产品说明）
蛋白名称：Human B7-H3 Protein (ECD, His Tag, Site-Specific AF488 Conjugation)
蛋白构建：A DNA sequence encoding the human B7-H3 (Q5ZPR3-1) (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.5e5 of 293-CAR cells expressing the anti-B7-H3 scFv can be directly stained with 100 μL (2 μL stock solution diluted to 100 μL FCM buffer) of AF 488-labeled recombinant human B7-H3 protein (Cat. No. 11188-H86H-SD) and the percentage of CAR-expressing cells can be detected by flow cytometry. Non-transduced 293 cells was used as a control (QC tested). 2.5e5 of PBMCs can be stained with anti-CD3 antibody and AF 488-labeled recombinant human B7-H3 protein (Cat. No. 11188-H86H-SD) and detected by flow cytometry (QC tested).
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 29
蛋白分子量：The recombinant human B7-H3 consists of AA Met1-Thr461 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 50 kDa.
蛋白NP号：Q5ZPR3-1
蛋白氨基酸序列：Met1-Thr461
蛋白标签：C-His
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文献和实验蛋白分离纯化概述（An overview of protein isolation）
chemical energy to mechanical motion. Similarly, living organisms such as the human body are complex machines made up of many interacting systems. Proteins constitute the majority of the working parts of these systems and there are thus diverse reasons
结合的单克隆抗体1。与Rho1D4抗体结合的表位可以作为针对膜蛋白的超高特异性纯化标记。通过基因修饰可在欲研究的目标（膜）蛋白C端加上Rho1D4标记。一旦加载上该序列，即可用装载有Rho1D4抗体的亲和基质去捕获目标蛋白，随后添加Rho1D4肽，通过竞争性结合基质抗体的方式来洗脱目标蛋白。采用这种方法，相比改变pH值等其他洗脱方式，更加地温和。图1：假设有3个跨膜域的膜蛋白。Rho1D4标签加到膜蛋白C端，它的序列是T-E-T-S-Q-V-A-P-A2） Rho1D4的历史Rho1D4的表位
are frequently liberated from the peptides. This is the case for phosphoserine- and phosphothreonine-containing peptides, which can liberate HPO3 or H3 PO4 , resulting in a neutral loss of 80 and 98 Da, respectively. Careful examination of the TOF spectrum
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