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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human MMP-3 Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
5.00 µg/20.00 µg/100.00 µg
| 规格: | 5.00 µg | 产品价格: | ¥750.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 100.00 µg | 产品价格: | ¥9080.0 |
蛋白名称:Human MMP-3 Protein
蛋白构建:A DNA sequence encoding the human MMP3 (AAA36321.1) N-terminal fragment (Tyr 18-Thr 272) was expressed and purified.
表达宿主:E. coli
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K(Dnp)-NH2, AnaSpec, Catalog # 27114. The specific activity is >300 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form)
蛋白内毒素:Please contact us for more information.
预测N端:Met 1
蛋白分子量:The recombinant human MMP3 consisting of 256 amino acids and has a calculated molecular mass of 29 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:AAA36321.1
蛋白氨基酸序列:Tyr18-Thr272
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Nuñez-delMoral A, et al. Characterization of Hevin (SPARCL1) Immunoreactivity in Postmortem Human Brain Homogenates.Neuroscience, PubMed ID: 34033869
3, Guo Y, et al. Integrating UHPLC-Q-TOF-MS/MS, network pharmacology, bioinformatics and experimental validation to uncover the anti-cancer mechanisms of TiaoPi AnChang decoction in colorectal cancer.Journal of ethnopharmacology, PubMed ID: 39002822
Expression of Recombinant MMP-28 in Mammalian Cells
The expression of a recombinant MMP in a mammalian cell line can be useful, e.g., for purification of the enzyme, to characterize function of the enzyme, or to uncover its substrates. In this chapter, we have therefore documented
Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System
Resident cells of tissues are capable of secreting an array of structurally related zinc endopeptidases known as matrix metalloproteinases (MMPs). They initiate the degradation of the surrounding macromolecules of the extracellular matrix
Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading activity. This polyacrylamide gel electrophoresis-based method can provide a reliable assessment of the type of gelatinase, relative amount, and activation status
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