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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human XRCC5 & XRCC6 Heterodimer Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/50.00 µg/500.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥1790.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥3870.0 |
| 规格: | 500.00 µg | 产品价格: | ¥25190.0 |
蛋白名称:Human XRCC5 & XRCC6 Heterodimer Protein
蛋白构建:A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:His & His
蛋白分子量:The recombinant heterodimer of human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号:P13010 & P12956
蛋白氨基酸序列:Met1-Ile732 & Met1-Asp609
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 询问日期
文献和实验2, Meza-Sosa, KF; et al. SPARCLE, a p53-induced lncRNA, controls apoptosis after genotoxic stress by promoting PARP-1 cleavage. Molecular cell, PMID: 35104452
3, Ning, K; et al. The small nonstructural protein NP1 of human bocavirus 1 directly interacts with Ku70 and RPA70 and facilitates viral DNA replication. PLoS pathogens, PMID: 35653410
4, Pan, Q; et al. PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair. The Journal of biological chemistry, PMID: 37437887
5, Wang, Y; et al. Homeodomain protein PRRX1 anchors the Ku heterodimers at DNA double-strand breaks to promote nonhomologous end-joining. Nucleic acids research, PMID: 40114375
6, Su, CH; et al. Phosphorylated Y14 condensates as a scaffold for DNA double-strand break repair. iScience, PMID: 40727937
白细胞介素-2(interleukin rhlL-2,IL-2)主要由活化的T淋巴细胞分泌的一种糖蛋白,是调节机体免疫功能最主要的淋巴因子之一。天然IL-2含有糖基,基因重组IL-2不含糖基,由133个氨基酸组成,相对分子质量为15KD。IL-2在机体应答,免疫调节和抗肿瘤免疫中具有十分重要的作用。临床用于多种恶性实体肿瘤。重组人IL-2是一种重组人白细胞介素-2的生物制品,一般制备成粉针剂应用,其规格以活性单位而不是以重量单位标示。本研究拟通过双抗体放射免疫分析法又称为免疫沉淀法测定注射
,重组人FGF-10(产品编号:100-26)需用5mMSodium Phosphate(磷酸钠),pH7.4溶解,重组IL-13(产品编号:200-13)需用20mMMHCl溶解。(注:即使同一重组细胞因子或蛋白,不同批次的溶解方法也可能有所不同,因此上面的叙述仅供参考。具体应该如何溶解应以相应批次的官方说明书为准)。后续的文章中将对各种溶解液的配制方法进行详细的阐述,望继续关注。 经常有用户会问,为什么会有这么多种溶解方法? 答:我们知道,蛋白的溶解性与很多因素有关,其中比
要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解,而重组人IL-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解,重组人FGF-10(产品编号:100-26)需用5mMSodium
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