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100 µg
蛋白名称:HER3/ERBB3蛋白, HER3/ERBB3 protein
蛋白构建:A DNA sequence encoding the mouse ERBB3 (Q61526) extracellular domain (Met 1-His 641) was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 93 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. 1. Immobilized mouse ErbB3-His (Cat: 51003-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (isoform Beta1) (Cat: 11609-H01H2) with a linear range of 0.018-0.125 μg/ml. 2. Immobilized mouse ErbB3-His (Cat: 51003-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (aa 2-246)-Fc (Cat: 11609-H04) with a linear range of 6.25-100 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 20
蛋白分子量:The secreted recombinant mouse ERBB3 comprises 633 amino acids and has a calculated molecular mass of 70 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 95 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q61526
蛋白氨基酸序列:Met1-His641
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
注意的是,两种配体的洗脱和再生buffer稍有差别,使用的时候还需注意! 系统特点 高达 pM 范围的亲和力(KD up to 6.2 x 10-11 M) 纯化树脂材质稳定 小标签 (8-28 aa)无需移除,不影响蛋白折叠和功能 极高的蛋白纯度(纯度>95%) 应用于多种目标蛋白检测系统:Western blot, ELISA, 免疫荧光, FACS等 兼容多种缓冲条件:高盐,洗涤剂,金属离子,螯合剂,还原剂 适用于多种蛋白的纯化 Twin-Strep-tag®标签蛋白固定在界面上时 (SPR
receptor) signaling pathway. The pathway consists of at least four Cell ular receptors: EGFR/HER1, HER2, HER3, and HER4. Approximately 11 different factors are known to bind and activate these receptors in certain patterns. The HER signaling pathway plays
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