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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse CD59a / Protectin / MAC-IP Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/50.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥1790.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:CD59a蛋白, CD59a protein
蛋白构建:A DNA sequence encoding the mouse CD59a (NP_031680.2) (Met 1-Lys 95), without the pro peptide, was fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 24
蛋白分子量:The secreted recombinant mouse CD59a comprises 83 amino acids and has a calculated molecular mass of 9.7 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_031680.2
蛋白氨基酸序列:Met1-Lys95
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验的,但是这结果是不是假阳性呢?还得做个阴性对照。阴性对照如何设置呢?考虑到整个实验体系会出现 beads、抗X抗体、诱饵蛋白 X、靶蛋白 Y(co-IP): 可能出现的假阳性来源 需要的阴性对照 抗 X 抗体结合非特异的蛋白 与 IP 一抗种属亚型相同的免疫球蛋白(比如,抗 X 抗体是 Mouse IgG,阴性对照可使用 Mouse 免疫球蛋白) beads 对蛋白的非特异结合* 同上,此外还可进行 Pre clear,IP 正式实验前排除非特异结合蛋白。如果使用琼脂
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
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