Recombinant Mouse Angiotensinogen / SerpinA8 / AGT Protein (His Tag) | 重组小鼠 Angiotensinogen / SerpinA8 / AGT 蛋白 (His标签)
- ￥3870
- Sino Biological
- 北京
- 50337-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse Angiotensinogen / SerpinA8 / AGT Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：Angiotensinogen蛋白, Angiotensinogen protein
蛋白构建：A DNA sequence encoding the mouse AGT (P11859) (Met 1-Val 477) was expressed, with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 25
蛋白分子量：The secreted recombinant mouse AGT consists of 464 amino acids and has a calculated molecular mass of 50.8 kDa. The apparent molecular mass of the recombinant mouse AGT is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P11859
蛋白氨基酸序列：Met1-Val477
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Göppner, C; et al. Pathogenesis of hypertension in a mouse model for human CLCN2 related hyperaldosteronism. Nat Commun, Pubmed: 31615979
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Arabidopsis protein microarrays. Proc. Natl. Acad. Sci. U.S.A. 104:4730‐4735. Ptacek, J., Devgan, G., Michaud, G., Zhu, H., Zhu, X., Fasolo, J., Guo, H., Jona, G., Breitkreutz
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Small Scale GST-Tag Purification Under Nature Conditions
of overloading). 3) Wash beads with 2x1.5ml PBS (washing: mix, spin 3min 3500rpm, keep supernatant aside). Keep sample of 40ul for PAGE-SDS of each washing . 4) Elute recombinant protein with 3 x 50ul elution buffer: 50mM TrisHCl pH8.0 10mM reduce
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