Recombinant Human CD3D & CD3E Heterodimer Protein (ECD)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
500 µg/50 µg
规格:
500 µg
产品价格:
¥13280
规格:
50 µg
产品价格:
¥3150
重组人 CD3D & CD3E 异源二聚体 蛋白 (ECD)(产品说明)
蛋白名称:Human CD3D & CD3E Heterodimer Protein (ECD)
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to bind OKT3-mIgG2a (Cat: 10977-M001) in a functional ELISA.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 22 & Asp 23
蛋白分子量:The recombinant heterodimer of human CD3D/CD3E consists of 687 (334+353) amino acids and predicts a molecular mass of 77.5 (37.7+39.8) kDa. It migrates as an approximately 44-54 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:P04234-1 & P07766-1
蛋白氨基酸序列:Met1-Ala105 & Met1-Asp126
蛋白标签:C-human IgG1-Fc & His & C-human IgG1-Fc & Flag
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Kahl, M; et al. Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts. Protein Expr. Purif., Pubmed: 29704557
2, Egan, TJ; et al. Novel multispecific heterodimeric antibody format allowing modular assembly of variable domain fragments. MAbs, Pubmed: 27786600
3, Zhou, Y; et al. A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer. J. Exp. Clin. Cancer Res., Pubmed: 32398042