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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CD3D & CD3E Heterodimer Protein (ECD)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/50.00 µg/500.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥2050.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥3150.0 |
| 规格: | 500.00 µg | 产品价格: | ¥13280.0 |
蛋白名称:Human CD3D & CD3E Heterodimer Protein (ECD)
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to bind OKT3-mIgG2a (Cat: 10977-M001) in a functional ELISA.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 22 & Asp 23
蛋白分子量:The recombinant heterodimer of human CD3D/CD3E consists of 687 (334+353) amino acids and predicts a molecular mass of 77.5 (37.7+39.8) kDa. It migrates as an approximately 44-54 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:P04234-1 & P07766-1
蛋白氨基酸序列:Met1-Ala105 & Met1-Asp126
蛋白标签:C-human IgG1-Fc & His & C-human IgG1-Fc & Flag
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Kahl, M; et al. Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts. Protein expression and purification, PMID: 29704557
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