- ￥2050 - 13280
- Sino Biological
- 北京
- CT026-H0323H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD3D & CD3E Heterodimer Protein (ECD)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg/500.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥2050.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥3150.0 |
| 规格： | 500.00 µg | 产品价格： | ￥13280.0 |
蛋白名称：Human CD3D & CD3E Heterodimer Protein (ECD)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to bind OKT3-mIgG2a (Cat: 10977-M001) in a functional ELISA.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Phe 22 & Asp 23
蛋白分子量：The recombinant heterodimer of human CD3D/CD3E consists of 687 (334+353) amino acids and predicts a molecular mass of 77.5 (37.7+39.8) kDa. It migrates as an approximately 44-54 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P04234-1 & P07766-1
蛋白氨基酸序列：Met1-Ala105 & Met1-Asp126
蛋白标签：C-human IgG1-Fc & His & C-human IgG1-Fc & Flag
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Kahl M, et al. Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts.Protein expression and purification, PubMed ID: 29704557
of T-cell activation: CD2, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, CD8A, CD8B1, CLECSF12, ICOSL, IRF4, KIF13B, NCK1, NCK2, PRLR, SIT, SLA2, TNFSF14. T-cell proliferation: CD28, CD3E, GLMN, ICOSL, IL10, IL12B, IL18, IL27, NCK1, NCK2, SFTPD, SPP
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
for examining in vitro proliferation of mouse splenic T-cells and human peripheral T cells stimulated via CD3. All steps where optimization by the user can be achieved for meaningful results have been highlighted. Briefly, these include titration of the cell
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