CD3D & CD3E Heterodimer重组蛋白|Recombinant Human CD3D & CD3E Heterodimer Protein (Flag & His Tag)
- ￥2310 - 25190
- Sino Biological
- 北京
- CT038-H2508H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD3D & CD3E Heterodimer Protein (Flag & His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg/500.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 500.00 µg | 产品价格： | ￥25190.0 |
蛋白名称：Human CD3D & CD3E Heterodimer Protein (Flag & His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein (Cat: CT038-H2508H) at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a (Cat: 10977-M001). The EC50 of OKT3-mIgG2a (Cat: 10977-M001) is 7-28 ng/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Phe 22 & Asp 23
蛋白分子量：The recombinant heterodimer of human CD3D&CD3E comprises 271 (126+145) amino acids and has a calculated molecular mass of 31.05 (14.4+16.65)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.
蛋白NP号：P04234-1 & P07766-1
蛋白氨基酸序列：Met1-Ala105 & Met1-Asp126
蛋白标签：C-Flag & C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验of T-cell activation: CD2, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, CD8A, CD8B1, CLECSF12, ICOSL, IRF4, KIF13B, NCK1, NCK2, PRLR, SIT, SLA2, TNFSF14. T-cell proliferation: CD28, CD3E, GLMN, ICOSL, IL10, IL12B, IL18, IL27, NCK1, NCK2, SFTPD, SPP
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
for examining in vitro proliferation of mouse splenic T-cells and human peripheral T cells stimulated via CD3. All steps where optimization by the user can be achieved for meaningful results have been highlighted. Briefly, these include titration of the cell
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