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20 µg/100 µg
| 规格: | 20 µg | 产品价格: | ¥3870 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5820 |
蛋白名称:MMP-8蛋白, MMP-8 protein
蛋白构建:A DNA sequence encoding the pro form of human MMP8 (NP_002415.1) (Phe 21-Gly 467) was expressed and purified, with an initial Met at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076) . The specific activity is > 250 pmoles/min/μg .(Activation description: The proenzyme needs to be activated by APMA for an activated form)
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human MMP8 consisting of 448 amino acids and has a calculated molecular mass of 52 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_002415.1
蛋白氨基酸序列:Phe21-Gly467
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验flower1946 菜鸟求助各位大虾,要做miRNA的实验,现已知可以查某个特定miRNA的有哪几个调节靶点,那反过来,如果要查调节某个特定蛋白(如MMP-7)的miRNA,该如何查呢?谢谢! zhujoker 可以使用targscan等软件啊,其实现在很多网站都有了,包括sigma也有一些预测 flower1946 感谢楼上 295546
Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading activity. This polyacrylamide gel electrophoresis-based method can provide a reliable assessment of the type of gelatinase, relative amount, and activation status
Assessment of Gelatinases (MMP-2 and MMP-9 by Gelatin Zymography
The invasion and metastasis of tumor cells has been shown to require proteolytic activity in order to degrade components of the extracellular matrix (ECM). The hydrolysis of the ECM appears to facilitate tumor cell migration contributing
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