mirVana miRNA isolation kit

The Ambion® mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)–based method. The method isolates total RNA ranging in size from kilobases down to 10mers. Each kit contains sufficient reagents for up to 40 purifications.

• Efficient isolation of small RNA–containing total RNA
• Enrich for small RNA <200 nt to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Efficient Recovery of Small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts