手机验证
Minute™ Lysosome Isolation Kit for mammalian cells/tissues
4℃
20 Preps
MinuteTM 动物细胞/组织溶酶体分离试剂盒
目录号:LY-034
描述 溶酶体是真核细胞中的球形囊泡,负责清除废物。溶酶体中的消化酶在消化多余或受损的细胞器、食物颗粒和吞噬的病毒和细菌中起着至关重要的作用。溶酶体是比较大的细胞器,大小从0.1到1.2μm不等。分离溶酶体是研究细胞自噬、蛋白质降解和蛋白质循环重要的第一步。传统的溶酶体分离方法是基于密度梯度超速离心法的。需要大量的起始材料,并且方法冗长且耗时,具有显著的交叉污染。目前,所有用于溶酶体分离的商业试剂盒都是基于上世纪70年代 发展起来的方法。我们的试剂盒与市场上的任何其他溶酶体分离试剂盒不同,使用专利技术的离心管柱,简单、快速、高效。所需的起始细胞/组织的量远小于传统方法。该试剂盒可以显著地富集培养细胞或组织中的溶酶体,而不使用杜恩斯匀浆器和超高速离心。整个操作过程可以在不到1.5小时内完成。1. Ravodina, A. M., Badgeley, M. A., Rajagopalan, S., Fedyukina, D. V., & Maiseyeu, A. (2020). Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays. ACS Omega.
2.Kim, J. W., Mahiddine, F. Y., & Kim, G. A. (2020). Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). International Journal of Molecular Sciences, 21(23), 8963.
3.Casey, C. A., Donohue Jr, T. M., Kubik, J. L., Kumar, V., Naldrett, M. J., Woods, N. T., ... & Thomes, P. G. (2021). Lipid droplet membrane proteome remodeling parallels ethanol-induced hepatic steatosis and its resolution.Journal of Lipid Research, 100049.
4.Jing, Z., Gao, J., Li, J., Niu, F., Tian, L., Nan, P., ... & Zhao, X. (2021). Acetylation-induced PCK isoenzyme transition promotes metabolic adaption of liver cancer to systemic therapy. Cancer Letters.
5.Gordevicius, J., Li, P., Marshall, L. L., Killinger, B. A., Lang, S., Ensink, E., ... & Labrie, V. (2021). Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease. Nature Communications, 12(1), 1-18.
6.Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.
7.Cao, X., Meng, P., Shao, Y., Yan, G., Yao, J., Zhou, X., ... & Lu, H. (2022). Nascent Glycoproteome Reveals That N-Linked Glycosylation Inhibitor-1 Suppresses Expression of Glycosylated Lysosome-Associated Membrane Protein-2. Frontiers in Molecular Biosciences, 9.
8.Tao, L., Qing, Y., Cui, Y., Shi, D., Liu, W., Chen, L., ... & Zhang, L. (2022). Lysosomal membrane permeabilization mediated apoptosis involve in perphenazine-induced hepatotoxicity in vitro and in vivo. Toxicology Letters.
9.Tsujimoto, K., Jo, T., Nagira, D., Konaka, H., Park, J. H., Yoshimura, S. I., ... & Kumanogoh, A. (2022). The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6. The EMBO Journal, e111389.
10.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
10.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
11.Qing, Y., Guo, Y., Zhao, Q., Hu, P., Li, H., Yu, X., ... & Hui, H. (2023). Targeting lysosomal HSP70 induces acid sphingomyelinase‐mediated disturbance of lipid metabolism and leads to cell death in T cell malignancies. Clinical and Translational Medicine, 13(3), e1229.
12.Y. Li, Z. Fan, Q. Jia, et al., Chaperone-mediated autophagy (CMA) alleviates cognitive impairment by reducing neuronal death in sepsis-associated encephalopathy (SAE), Experimental Neurology (2023), https://doi.org/10.1016j.expneurol.2023.114417
13.Ren, G., Ding, Y. W., Wang, L. L., & Jiang, J. D. (2023). Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1. Frontiers in Pharmacology, 14.
14.Secker, C., Motzny, A. Y., Kostova, S., Buntru, A., Helmecke, L., Reus, L., ... & Wanker, E. E. (2023). The polyphenol EGCG directly targets intracellular amyloid‐β aggregates and promotes their lysosomal degradation. Journal of Neurochemistry.
15.Lin, C. P., Wu, S. H., Lin, T. Y., Chu, C. H., Lo, L. W., Kuo, C. C., ... & Huang, D. M. Lysosomal-Targeted Doxorubicin Delivery Using Rbc-Derived Vesicles to Overcome Drug-Resistant Cancer Through Mitochondrial-Dependent Cell Death. Available at SSRN 4492777.
16.Chen, L., Ma, Y., Ma, X., Liu, L., Jv, X., Li, A., ... & Xie, J. (2023). TFEB regulates cellular labile iron and prevents ferroptosis in a TfR1-dependent manner. Free Radical Biology and Medicine.
17.Morishige, J. I., Yoshioka, K., Nakata, H., Ishimaru, K., Nagata, N., Tanaka, T., ... & Ando, H. (2023). Sphingosine Kinase 1 is Involved in Triglyceride Breakdown by Maintaining Lysosomal Integrity in Brown Adipocytes. Journal of Lipid Research, 100450.
18.He, L., Chen, J., Deng, P., Huang, S., Liu, P., Wang, C., ... & Lin, C. (2023). Lysosomal cyst (e) ine storage potentiates tolerance to oxidative stress in cancer cells. Molecular Cell.
19.Wang, Z., He, W., Li, C., Chen, Y., Li, Z., Jiao, Y., ... & Lan, Y. (2023). The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. Journal of Virology, e01338-23.
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1. Ravodina, A. M., Badgeley, M. A., Rajagopalan, S., Fedyukina, D. V., & Maiseyeu, A. (2020). Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays. ACS Omega.
2.Kim, J. W., Mahiddine, F. Y., & Kim, G. A. (2020). Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). International Journal of Molecular Sciences, 21(23), 8963.
3.Casey, C. A., Donohue Jr, T. M., Kubik, J. L., Kumar, V., Naldrett, M. J., Woods, N. T., ... & Thomes, P. G. (2021). Lipid droplet membrane proteome remodeling parallels ethanol-induced hepatic steatosis and its resolution.Journal of Lipid Research, 100049.
4.Jing, Z., Gao, J., Li, J., Niu, F., Tian, L., Nan, P., ... & Zhao, X. (2021). Acetylation-induced PCK isoenzyme transition promotes metabolic adaption of liver cancer to systemic therapy. Cancer Letters.
5.Gordevicius, J., Li, P., Marshall, L. L., Killinger, B. A., Lang, S., Ensink, E., ... & Labrie, V. (2021). Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease. Nature Communications, 12(1), 1-18.
6.Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.
7.Cao, X., Meng, P., Shao, Y., Yan, G., Yao, J., Zhou, X., ... & Lu, H. (2022). Nascent Glycoproteome Reveals That N-Linked Glycosylation Inhibitor-1 Suppresses Expression of Glycosylated Lysosome-Associated Membrane Protein-2. Frontiers in Molecular Biosciences, 9.
8.Tao, L., Qing, Y., Cui, Y., Shi, D., Liu, W., Chen, L., ... & Zhang, L. (2022). Lysosomal membrane permeabilization mediated apoptosis involve in perphenazine-induced hepatotoxicity in vitro and in vivo. Toxicology Letters.
9.Tsujimoto, K., Jo, T., Nagira, D., Konaka, H., Park, J. H., Yoshimura, S. I., ... & Kumanogoh, A. (2022). The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6. The EMBO Journal, e111389.
10.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
10.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
11.Qing, Y., Guo, Y., Zhao, Q., Hu, P., Li, H., Yu, X., ... & Hui, H. (2023). Targeting lysosomal HSP70 induces acid sphingomyelinase‐mediated disturbance of lipid metabolism and leads to cell death in T cell malignancies. Clinical and Translational Medicine, 13(3), e1229.
12.Y. Li, Z. Fan, Q. Jia, et al., Chaperone-mediated autophagy (CMA) alleviates cognitive impairment by reducing neuronal death in sepsis-associated encephalopathy (SAE), Experimental Neurology (2023), https://doi.org/10.1016j.expneurol.2023.114417
13.Ren, G., Ding, Y. W., Wang, L. L., & Jiang, J. D. (2023). Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1. Frontiers in Pharmacology, 14.
14.Secker, C., Motzny, A. Y., Kostova, S., Buntru, A., Helmecke, L., Reus, L., ... & Wanker, E. E. (2023). The polyphenol EGCG directly targets intracellular amyloid‐β aggregates and promotes their lysosomal degradation. Journal of Neurochemistry.
15.Lin, C. P., Wu, S. H., Lin, T. Y., Chu, C. H., Lo, L. W., Kuo, C. C., ... & Huang, D. M. Lysosomal-Targeted Doxorubicin Delivery Using Rbc-Derived Vesicles to Overcome Drug-Resistant Cancer Through Mitochondrial-Dependent Cell Death. Available at SSRN 4492777.
16.Chen, L., Ma, Y., Ma, X., Liu, L., Jv, X., Li, A., ... & Xie, J. (2023). TFEB regulates cellular labile iron and prevents ferroptosis in a TfR1-dependent manner. Free Radical Biology and Medicine.
17.Morishige, J. I., Yoshioka, K., Nakata, H., Ishimaru, K., Nagata, N., Tanaka, T., ... & Ando, H. (2023). Sphingosine Kinase 1 is Involved in Triglyceride Breakdown by Maintaining Lysosomal Integrity in Brown Adipocytes. Journal of Lipid Research, 100450.
18.He, L., Chen, J., Deng, P., Huang, S., Liu, P., Wang, C., ... & Lin, C. (2023). Lysosomal cyst (e) ine storage potentiates tolerance to oxidative stress in cancer cells. Molecular Cell.
19.Wang, Z., He, W., Li, C., Chen, Y., Li, Z., Jiao, Y., ... & Lan, Y. (2023). The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. Journal of Virology, e01338-23.
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