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LY-034 Minute™ 动物细胞/组织溶酶体分离试剂盒

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  • ¥6282
  • Invent已认证
  • LY-034
  • 美国
  • 2025年12月30日
    48h发货
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    • 文献和实验
    • 技术资料
    • 英文名

      Minute™ Lysosome Isolation Kit for mammalian cells/tissues

    • 保存条件

      4℃

    • 规格

      20 Preps

    MinuteTM 动物细胞/组织溶酶体分离试剂盒

     

    目录号:LY-034

      描述   溶酶体是真核细胞中的球形囊泡,负责清除废物。溶酶体中的消化酶在消化多余或受损的细胞器、食物颗粒和吞噬的病毒和细菌中起着至关重要的作用。溶酶体是比较大的细胞器,大小从0.1到1.2μm不等。分离溶酶体是研究细胞自噬、蛋白质降解和蛋白质循环重要的第一步。传统的溶酶体分离方法是基于密度梯度超速离心法的。需要大量的起始材料,并且方法冗长且耗时,具有显著的交叉污染。目前,所有用于溶酶体分离的商业试剂盒都是基于上世纪70年代 发展起来的方法。我们的试剂盒与市场上的任何其他溶酶体分离试剂盒不同,使用专利技术的离心管柱,简单、快速、高效。所需的起始细胞/组织的量远小于传统方法。该试剂盒可以显著地富集培养细胞或组织中的溶酶体,而不使用杜恩斯匀浆器和超高速离心。整个操作过程可以在不到1.5小时内完成。

    产品细节图片1 产品细节图片2 产品细节图片3 产品细节图片4

    试剂盒组份 (20 preps):
    1. Buffer A 15ml
    2. Buffer B 2ml
    3. 塑料研磨棒 2 个
    4. 离心管柱 20 个
    5. 接收管 20 个

    重要产品信息:
    1. 操作前请仔细阅读整个操作说明。将离心管柱(Filter Cartridges)套入接收管成为套管放置于冰上预冷。
    2. 所有离心步骤都需要在 4℃室温下或者低温离心机中进行。
    3. 对于蛋白磷酸化研究,磷酸酶抑制剂务必在使用前加入缓冲液 A 中。如果担心蛋白质降解,使用前可在 Buffer A 和 Buffer B 中添加蛋白酶抑制剂。(请按照蛋白酶或磷酸酶抑制剂母液比例添加,例如母液是100X,添加时按照 1:100 添加,即 1ml 缓冲液中添加 10ul 抑制剂)
    4. 推荐使用 BCA 法进行蛋白浓度测定。
    5. 请注意,分离的溶酶体产量和纯度可能因具体细胞/组织类型和所用起始样品的量而异。您可能需要优化操作以获得最佳结果(具体优化操作请参阅下面的技术说明)

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      图标文献和实验
      该产品被引用文献

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      9.Tsujimoto, K., Jo, T., Nagira, D., Konaka, H., Park, J. H., Yoshimura, S. I., ... & Kumanogoh, A. (2022). The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6. The EMBO Journal, e111389.

      10.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

      11.Qing, Y., Guo, Y., Zhao, Q., Hu, P., Li, H., Yu, X., ... & Hui, H. (2023). Targeting lysosomal HSP70 induces acid sphingomyelinase‐mediated disturbance of lipid metabolism and leads to cell death in T cell malignancies. Clinical and Translational Medicine, 13(3), e1229.

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      14.Secker, C., Motzny, A. Y., Kostova, S., Buntru, A., Helmecke, L., Reus, L., ... & Wanker, E. E. (2023). The polyphenol EGCG directly targets intracellular amyloid‐β aggregates and promotes their lysosomal degradation. Journal of Neurochemistry.

      15.Lin, C. P., Wu, S. H., Lin, T. Y., Chu, C. H., Lo, L. W., Kuo, C. C., ... & Huang, D. M. Lysosomal-Targeted Doxorubicin Delivery Using Rbc-Derived Vesicles to Overcome Drug-Resistant Cancer Through Mitochondrial-Dependent Cell Death. Available at SSRN 4492777.

      16.Chen, L., Ma, Y., Ma, X., Liu, L., Jv, X., Li, A., ... & Xie, J. (2023). TFEB regulates cellular labile iron and prevents ferroptosis in a TfR1-dependent manner. Free Radical Biology and Medicine.

      17.Morishige, J. I., Yoshioka, K., Nakata, H., Ishimaru, K., Nagata, N., Tanaka, T., ... & Ando, H. (2023). Sphingosine Kinase 1 is Involved in Triglyceride Breakdown by Maintaining Lysosomal Integrity in Brown Adipocytes. Journal of Lipid Research, 100450.

      18.He, L., Chen, J., Deng, P., Huang, S., Liu, P., Wang, C., ... & Lin, C. (2023). Lysosomal cyst (e) ine storage potentiates tolerance to oxidative stress in cancer cells. Molecular Cell.

      19.Wang, Z., He, W., Li, C., Chen, Y., Li, Z., Jiao, Y., ... & Lan, Y. (2023). The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. Journal of Virology, e01338-23.

      20.Jiang Y, Chen X, Ye X, Wen C, Xu T, Yu C, Ning W, Wang G, Xiang X, Liu X, Wang Y, Chen Y, Liu X, Shi C, Liu C, Yuan Q, Chen Y, Zhang T, Luo W, Xia N. A Dual-domain Engineered Antibody for Efficient HBV Suppression and Immune Responses Restoration. Adv Sci (Weinh). 2024 Feb 11:e2305316. doi: 10.1002/advs.202305316. Epub ahead of print. PMID: 38342604.

      21.Wang, S., Li, B., Cai, Z., Hugo, C., Li, J., Sun, Y., ... & Yassine, H. (2024). Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD.

      22.Qin, J., Huang, X., Gou, S., Zhang, S., Gou, Y., Zhang, Q., ... & Xue, Y. (2024). Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation. Nature Metabolism, 1-24.

      23.L. Yang, Y. Huang, Y. Peng, Q. Sun, D. Zhang, S. Yang, J. Song, X. Sun, C. Lv, X. Zhang, Z. Fang, Chaperone-mediated autophagy (CMA) confers neuroprotection of HBO preconditioning against stroke, Brain Research (2024), doi: doi.org/10.1016/j.brainres.2024.149315

      24.Qian Liu, Xin Wang, Zhi-Ting Fang, Jun-Ning Zhao, Xue-Xiang Rui,Bing-Ge Zhang, Ye He, Rui-Juan Liu, Jian Chen, Gao-Shang Chai & Gong-Ping Liu.(2024).Upregulation of ISG15 induced by MAPT/tau accumulation represses autophagic flux by inhibiting HDAC6 activity: a vicious cycle in alzheimer disease, Autophagy, DOI: 10.1080/15548627.2024.2431472
      25.Meirui Qian; Ziyu Wan; Xue Liang; Jing Lin; Huijie Zhang; et al.(2024). Targeting autophagy in HCC treatment: exploiting the CD147 internalization pathway.Cell Communication and Signaling.DOI 10.1186/s12964-024-01956-5

      26. Qingling Fan; Guanya Guo; Yinan Hu; Yi Lu; Ruifeng Su; et al.(2025).Hepatic Lamp2a Deficiency Promotes inflammation of Murine Autoimmune Cholangitis via Affecting Bile Acid Metabolism. iScience.DOI 10.1016/j.isci.2025.111804

      27.Yu Zhang; Wei Su; Xiaoyu Ji; Yang Zhou; Qing Guan; et al.(2025).PCSK9 promotes progression of anaplastic thyroid cancer through E-cadherin endocytosis.Cell Death and Disease.DOI 10.1038/s41419-025-07690-1

      28.H. Feng, J. Wang, L. Tao, L. Li, M. Fan, C. Yu, D. Sun, H. Cheng, W. Shen,(2025).α-hederin decreases the glycolysis level in intestinal epithelial cells via SNX10-mediated DEPDC5 degradation, Journal of Pharmaceutical Analysis, DOI 10.1016/j.jpha.2025.101301

      29.Yi Ju; Yuting Zhang; Xiaolin Tian; Ningwen Zhu; Yufan Zheng; et al.(2025).Protein S-glutathionylation confers cellular resistance to ferroptosis induced by glutathione depletion. Redox Biology.DOI 10.1016/j.redox.2025.103660

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      研选
      LY-034 Minute™ 动物细胞/组织溶酶体分离试剂盒
      ¥6282