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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase(YNK1)
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:Greater than 85% as determined by SDS-PAGE.
other_names:NDK1, YNK
accession_no:P36010
calculated_mw:37.2 kDa
tag info:N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:1-153aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Required for repair of UV radiation- and etoposide-induced DNA damage.
产品详细信息请点击:Recombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase(YNK1)
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
., Rushing, K.L., Jackson, C.H., Lockshon, D., Conover, D., Lanciault, C., Harris, J.R., Simmons, S.J., Rothstein, R., and Fields, S. 1997. The complete set of predicted genes from Saccharomyces cerevisiae in a readily usable form. Genome Res. 7:1169‐1173
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
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Introduction of human 14-3-3 proteins
-binding complex.Previously,we isolated the BMH1 gene from Saccharomyces cerevisiae encoding a putative 14-3-3 protein.Using the polymerase chain reaction method,we have isolated a second yeast gene encoding a 14-3-3 protein (BMH2).While disruption
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