- ¥2980
- LMAI Bio
- LM-13190R-FITC
- 中国/美国/欧洲
- 2025年07月15日
- ICC=1:50-200 IF=1:50-200
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-13190R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-FNBP3/FITC
- 抗体名：
Anti-FNBP3/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
ICC=1:50-200 IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-FNBP3/FITC |
| 中文名称 | FITC标记的甲精结合蛋白3抗体 |
| 别 名 | Fas ligand associated factor 1; Fas ligand-associated factor 1; FBP 11; FBP11; FLAF 1; FLAF1; FNBP 3; Formin binding protein 11; Formin binding protein 3; Formin-binding protein 11; Formin-binding protein 3; HIP-10; HIP10; Huntingtin interacting protein A; Huntingtin interacting protein HYPA; Huntingtin yeast partner A; Huntingtin-interacting protein 10; Huntingtin-interacting protein A; HYPA; NY REN 6; NY REN 6 antigen; PR40A_HUMAN; Pre mRNA processing factor 40 homolog A; Pre-mRNA-processing factor 40 homolog A; PRP40 pre mRNA processing factor 40 homolog A (S cerevisiae); PRP40 pre mRNA processing factor 40 homolog A (yeast); PRP40 pre mRNA processing factor 40 homolog A; PRPF 40A; PRPF40A; Renal carcinoma antigen NY-REN-6. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 转运蛋白 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 109kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FNBP3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Fas ligand associated factor 1; Fas ligand-associated factor 1; FBP 11; FBP11; FLAF 1; FLAF1; FNBP 3; Formin binding protein 11; Formin binding protein 3; Formin-binding protein 11; Formin-binding protein 3; HIP-10; HIP10; Huntingtin interacting protein A; Huntingtin interacting protein HYPA; Huntingtin yeast partner A; Huntingtin-interacting protein 10; Huntingtin-interacting protein A; HYPA; NY REN 6; NY REN 6 antigen; PR40A_HUMAN; Pre mRNA processing factor 40 homolog A; Pre-mRNA-processing factor 40 homolog A; PRP40 pre mRNA processing factor 40 homolog A (S cerevisiae); PRP40 pre mRNA processing factor 40 homolog A (yeast); PRP40 pre mRNA processing factor 40 homolog A; PRPF 40A; PRPF40A; Renal carcinoma antigen NY-REN-6. Function: Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). May be involved in pre-mRNA splicing. Subunit: Interacts with the N-terminus of HTT. Interacts with the phosphorylated C-terminal domain of POLR2A. Interacts with AKAP8L, SF1, SRPK1, CARD8, ATBF1 and MECP2 (By similarity). Interacts through the WW domains with formin proline-rich regions and with WASL/N-WASP (By similarity). Subcellular Location: Nucleus speckle. Nucleus matrix. Colocalizes with AKAP8L in the nuclear matrix. Tissue Specificity: Expressed in the brain cortex (at protein level). Widely expressed. Similarity: Belongs to the PRPF40 family. Contains 5 FF domains. Contains 2 WW domains. Database links: UniProtKB/Swiss-Prot: O75400.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物，过葡萄糖凝胶G-25或G-50柱，分离出游离荧光素，收集标记的荧光抗体进行鉴定。
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