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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-19461R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
PSMA8
- 抗体名:
蛋白酶体PSMα8抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Cynomolgus Monkey, Rhesus monkey, Opossum
- 免疫原:
KLH conjugated synthetic peptide derived from human PSMA8:121-220/256
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | PSMA8 |
| 中文名称 | 蛋白酶体PSMα8抗体 |
| 别 名 | MGC26605; Proteasome subunit alpha type 7 like; Proteasome subunit alpha type-7-like; PSA7L_HUMAN; PSMA 7L; PSMA 8; PSMA7L; PSMA8; PSMA8 proteasome (prosome macropain) subunit alpha type 8; PSMA8 proteasome subunit alpha type 8. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Cynomolgus Monkey, Rhesus monkey, Opossum |
| 产品应用 | WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 29kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PSMA8:121-220/256 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity (By similarity). This may be a testis-specific subunit. Subunit: The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the peptidase T1A family. SWISS: Q8TAA3 Gene ID: 143471 Database links: Entrez Gene: 143471 Human Entrez Gene: 73677 Mouse SwissProt: Q8TAA3 Human SwissProt: Q9CWH6 Mouse Unigene: 464813 Human Unigene: 87277 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: TM4(Mouse) Cell Lysate at 30 ug Primary: Anti-PSMA8 (bs-19461R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD ![]() Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMA8) Polyclonal Antibody, Unconjugated (bs-19461R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. ![]() Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMA8) Polyclonal Antibody, Unconjugated (bs-19461R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 26S 蛋白酶体 (26S PSM)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 26S PSM 单抗包被于酶标板上,标准品和样品中的 26S PSM 与单抗结合,加入生物素化的抗人 26S PSM
特异性使免疫测定能在一非常复杂的蛋白质化合物(例如血清)中测定某一特定的物质,而不需先分离待检物。 但是这种特异性也不是绝对的。假使两种化合物有着部分相同的结构,在抗原抗体反应中可出现交叉反应。例如:绒毛膜促性腺激素(hCG)和黄体生成激素(LH)均由 α 和 β 两个亚单位组成,其结构的不同处在 β 亚单位,而两者的 α 亚单位是同类的。用 hCG 免疫动物所得的抗血清中含有抗 α-hCG 和抗 β-hCG两种抗体,抗 α-hCG 抗体将与LH中的 α 酶位发生交叉反应。在临床检验中,如用抗 hCG
颌下腺粘蛋白 submaxillary gland mucin
成分。牛颌下腺粘蛋白以同样的二糖侧链为主体,这点与 OSM相似;猪颌下腺粘蛋白( porcin submaxillary gland mucin, PSM)的寡糖侧链结构,甚为复杂,是由两个 N-乙酰氨基半乳糖分别与 1个半乳糖、岩藻糖、唾液酸( N-羟乙酰神经氨(糖)酸—— N-glycolyl neuramin-ate)结合而构成的五糖。 PSM作为血型物质也具有活性( A型)。
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