- 询价
- gelatins
- JLC260734
- 上海
- 2025年07月10日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-GEMIN2 antibody
- 宿主：
Rabbit
- 浓度：
0.3 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC260734
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-GEMIN2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human GEMIN2
Reactivity Human, Mouse, Rat
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol GEMIN2
Full name gem (nuclear organelle) associated protein 2
Synonyms SIP1, SIP1-delta
Swissprot O14893
Target Background
This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human ovarian cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml260734(GEMIN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml260734(GEMIN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:32 kDa
Positive control:K562 and hela cells
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-2: K562 cells, hela cells
Primary antibody: ml260734(GEMIN2 Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 1000-5000
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