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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-GNRHR antibody
- 宿主:
Rabbit
- 浓度:
0.72 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-GNRHR antibody
Cat. No. JLC260462
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-GNRHR rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human GNRHR
Reactivity Human, Mouse
Content 0.72 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol GNRHR
Full name gonadotropin releasing hormone receptor
Synonyms HH7; GRHR; LRHR; LHRHR; GNRHR1
Swissprot P30968
Target Background
This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene.

Applications
Immunohistochemistry
Predicted cell location: Cell membrane
Positive control: Human tonsil
Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml260462(GNRHR Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:38 kDa
Positive control:PC-3 and LOVO cell lysates
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: PC-3 and LOVO cell lysates
Primary antibody: ml260462(GNRHR Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 3 seconds
ELISA
Recommended dilution: 5000-10000
Cat. No. JLC260462
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-GNRHR rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human GNRHR
Reactivity Human, Mouse
Content 0.72 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol GNRHR
Full name gonadotropin releasing hormone receptor
Synonyms HH7; GRHR; LRHR; LHRHR; GNRHR1
Swissprot P30968
Target Background
This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene.

Applications
Immunohistochemistry
Predicted cell location: Cell membrane
Positive control: Human tonsil
Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml260462(GNRHR Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:38 kDa
Positive control:PC-3 and LOVO cell lysates
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: PC-3 and LOVO cell lysates
Primary antibody: ml260462(GNRHR Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 3 seconds
ELISA
Recommended dilution: 5000-10000
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Anti-GNRHR antibody
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