Product overview Description Anti-VWA5A rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human VWA5A Reactivity Human Content 0.5 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol VWA5A Full name von Willebrand factor A domain containing 5A Synonyms BCSC1; BCSC-1; LOH11CR2A Swissprot O00534
Target Background VWA5A (von Willebrand factor A domain containing 5A), also known as BCSC-1 (breast cancer suppressor candidate 1) or LOH11CR2A (loss of heterozygosity 11 chromosomal region 2 gene A protein), is a 786 amino acid protein containing one VIT domain and one VWFA domain. VWA5A is expressed at low levels in various tissues, with no expression found in 80% of tumor cell lines. Likely acting as a tumor suppressor gene, deletion of VWA5A leads to loss of heterozygosity (LOH) in breast and ovarian tumors, and may have an important role as a potential gene therapy target. Abnormal expression of VWA5A may lead to an increase in adhesion of CNE-2L2 cells associated with an increase in expression of E-cadherin, alpha-catenin, and p53, resulting in a decrease of malignant activity in cells with ectopic expression of VWA5A.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human lung cancer Recommended dilution: 100-300 Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 100-300
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml221980(VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221980(VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:86 kDa Positive control:Human Adrenal pheochromocytoma tissue,Jurkat and A549 cells
Recommended dilution: 500-2000 Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-3: Human Adrenal pheochromocytoma tissue, Jurkat cells, A549 cells Primary antibody: ml221980(VWA5A Antibody) at dilution 1/275 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds