- 询价
- gelatins
- JLC221980
- 上海
- 2025年07月07日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-VWA5A antibody
- 宿主：
Rabbit
- 浓度：
0.5 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC221980
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-VWA5A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human VWA5A
Reactivity Human
Content 0.5 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol VWA5A
Full name von Willebrand factor A domain containing 5A
Synonyms BCSC1; BCSC-1; LOH11CR2A
Swissprot O00534
Target Background
VWA5A (von Willebrand factor A domain containing 5A), also known as BCSC-1 (breast cancer suppressor candidate 1) or LOH11CR2A (loss of heterozygosity 11 chromosomal region 2 gene A protein), is a 786 amino acid protein containing one VIT domain and one VWFA domain. VWA5A is expressed at low levels in various tissues, with no expression found in 80% of tumor cell lines. Likely acting as a tumor suppressor gene, deletion of VWA5A leads to loss of heterozygosity (LOH) in breast and ovarian tumors, and may have an important role as a potential gene therapy target. Abnormal expression of VWA5A may lead to an increase in adhesion of CNE-2L2 cells associated with an increase in expression of E-cadherin, alpha-catenin, and p53, resulting in a decrease of malignant activity in cells with ectopic expression of VWA5A.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 100-300 Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 100-300
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml221980(VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221980(VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:86 kDa
Positive control:Human Adrenal pheochromocytoma tissue,Jurkat and A549 cells
Recommended dilution: 500-2000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-3: Human Adrenal pheochromocytoma tissue, Jurkat cells, A549 cells
Primary antibody: ml221980(VWA5A Antibody) at dilution 1/275
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 2000-10000
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