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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Human COMP ELISA Kit
- 保存条件：
Human COMP ELISA Kit
- 库存：
软骨寡聚基质蛋白(COMP)检测试剂盒
- 供应商：
软骨寡聚基质蛋白(COMP)检测试剂盒
- 规格：
96T
Human COMP ELISA Kit
Sensitivity
1.1 pg/mL
Range
31.25 - 2000 pg/mL
Reactivity
Human
Cat No : KE00105
Synonyms
COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5
Product Information
KE00105 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The COMP ELISA kit is to be used to detect and quantify protein levels of endogenous COMP. The assay recognizes human COMP. An antibody specific for COMP has been pre-coated onto the microwells. The COMP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human COMP is added to detect the captured human COMP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing su lfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human COMP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 31.25 - 2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1311 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 76%-127% |
| Cell culture supernatants | 108% | 73%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 818.1 | 70.1 | 8.6 |
| 2 | 20 | 194.2 | 18.3 | 9.4 |
| 3 | 20 | 51.5 | 4.6 | 9.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 874.3 | 81.0 | 9.3 |
| 2 | 24 | 206.0 | 20.5 | 9.9 |
| 3 | 24 | 44.2 | 4.4 | 9.9 |
Background Information
COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5 |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 软骨寡聚基质蛋白 ( COMP )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 COMP 单抗包被于酶标板上，标准品和样品中的 COMP与单抗结合，加入生物素化的抗人 COMP ，形成
人 软骨寡聚基质蛋白 ( COMP )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 COMP 单抗包被于酶标板上，标准品和样品中的 COMP与单抗结合，加入生物素化的抗人 COMP ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 软骨寡聚基质蛋白 (COMP)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 COMP 单抗包被于酶标板上，标准品和样品中的 COMP 与单抗结合，加入生物素化的抗大鼠 COMP ，形成免疫复合物连接在板上
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