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- 文献和实验
- 技术资料
- 库存：
13512172575
- 英文名：
Internal Standard Solution Mix16
- 保存条件：
询QQ：1916572412
- 保质期：
13512172575
- 供应商：
西宝生物
- 规格：
1 mL x 2000 µg/mL (methanol)
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文献和实验Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Quantitative PCR can be done either by measuring the amount of PCR products at a given number of cycle (end-point quantitative PCR) (1 –4 ) or by following the amount of products during the PCR at several cycles (kinetic quantitative PCR
Prepare a ligation mix: Ligation Mix (2x
; lower for plasmid / purified DNA / virus DNA target)Buffer: use proprietary or home-made 10x rxn mix; eg: Cetus, Promega. This should contain: minimum of 1.5mM Mg2+, usually some detergent, perhaps some gelatin or BSA. Promega now supply 25mM MgCl
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