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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
询QQ:1916572479
- 库存:
13512172575
- 英文名:
Internal Standard Mix 35
- 供应商:
西宝生物
- 保质期:
13512172575
- 规格:
1 mL x 2000 µg/mL (toluene)
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文献和实验Standard Protocols Autoradiography (35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42o C for around 30-60 mins (until melted). Make up the developer and the fixer and place in the darkroom ready for later (only if developing the same day
Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Quantitative PCR can be done either by measuring the amount of PCR products at a given number of cycle (end-point quantitative PCR) (1 –4 ) or by following the amount of products during the PCR at several cycles (kinetic quantitative PCR
) by incorporating the reagent to 10-35% final effective dTTP concentration in a nucleotide mix of final concentration 50-100uM dNTPs (Emanual, 1991; Nucleic Acids Res 19: 2790). This allows substitution to a known extent of probes of exactly defined length
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