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文献和实验This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison. The ups and downs of various methods from sonication, homogenization, freeze-thaw cycles
细胞裂解液(Cell Lysis Solution)的配置方法
1.1M Tris 溶液的配置 取Tris242.2g,加ddH2O至1600ml,加热溶解。 2.1M Tris-HCL Ph=8.0溶液的配置 取 1M Tris 溶液160ml用分析纯盐酸调至Ph=8.0(需浓盐酸约8.5ml),加ddH2O定容至200ml,高压灭菌备用。 3. 0.5M EDTA溶液的配置 称EDTA-Na2 37.2g 先用140ml ddH2O溶解,加入14ml NaOH(10M)使EDTA-Na2溶解,再用NaOH(10M)溶液调至Ph
Cell Lysis/Western/IPSteven Finkbeiner,Departments of Neurology and Physiology,UCSFcompiled by 6/95 by AJS from AB)Fresh 1X LysisFresh 1X LysisFinal5ml10ml2X Lysis 2.5 ml5mlNaCl0.25M0.25ml of 5M0.5 ml of 5MNa3VO40.1mM25l (μl) of 200 mM5l (μl) of 200
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