T4 Polynucleotide Kinase, Clon

Polynucleotide Kinase (EC 2.7.1.78)

The enzyme polynucleotide kinase (PNK, or ATP:5′-dephospho-polynucleotide 5′-phosphatase) catalyzes the transfer of a γ-phosphate group from a 5′-nucleoside triphosphate moiety to a free 5′-hydroxyl of a polynucleotide such as DNA or RNA, to form

Radiolabeling of DNA Using Polynucleotide Kinase

Labeling of DNA fragments at the 5′ end with polynucleotide kinase is one of the methods currently employed to obtain highly labeled DNA for sequencing purposes. This end-labeled DNA is used in the partial chemical degradation method

多核苷酸激酶 polynucleotide kinase

　　由 T偶数噬菌体所感染的大肠杆菌分离出来的酶，能催化 ATP的γ -磷酸转移到 DNA或 RNA的 5′ -OH末端生成 ADP的反应。 EC2． 7． 1． 78。因为此酶的催化反应特异性很高，可用 32 P标记的 ATP特异地标记多核苷酸的 5′末端。广泛应用于多核苷酸的链长的测定和 5′末端核苷酸排列的确定等核酸结构的研究。