• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Cell and tissue lysis hub

        互联网

        1088

         

        This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison. The ups and downs of various methods from sonication, homogenization, freeze-thaw cycles, and detergent-based lysis are examined below.

        Comparison of lysis methods

         

        Sonication

        material: ultrasonication bath or rods

         

        Homogenisation

        material: drills (polytron), pestle/tube (dounce) bead beaters

         

        Freeze-thaw

        material: pestle and mortar, liquid nitrogen

         

        Detergents

        no additional material required, just the chemicals and typical tubes

         

        Specific protocols

         

        target    lysis method    type of material   
        Silver: Lysate for Western protein detergent (Triton) cells
        Sauer:Lysing E. coli with Lysozymes   lysozyme bacteria
        Blackburn:Yeast Colony PCR DNA NaOH yeast
        Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer protein detergent (RIPA buffer) cells
        Streptomyces:Protocols/Mini-Maxi Prep DNA NaOH bacteria
        Western Blot/Tissue Preparation protein detergent & physical disruption tissue
        Eccles:Protein Lysates from Cells in Culture protein detergent (SDS, NP40) cells
        Eccles:Protein Lysates from Tissue protein detergent, physical, freezing tissue
        RNA extraction using trizol/tri RNA phenol cells
        Sauer:RNA Purification from E. coli RNA various options bacteria
        add another method''s name target lysis method type of starting material

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序