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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-CALCOCO1 antibody
- 宿主:
Rabbit
- 浓度:
0.84 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl

Anti-CALCOCO1 antibody
Cat. No. JLC225479
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CALCOCO1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human CALCOCO1
Reactivity Human, Mouse, Rat
Content 0.84 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CALCOCO1
Full name calcium binding and coiled-coil domain 1
Synonyms Cocoa; PP13275; calphoglin
Swissprot Q9P1Z2
Target Background
CALCOCO1 (calcium-binding and coiled-coil domain-containing protein 1), also known as cocoa, calphoglin, sarcoma antigen NY-SAR-3 or coiled-coil coactivator protein, is a 691 amino acid protein that shuttles between the cytoplasm and nucleus and functions as coactivator for aryl hydrocarbon and nuclear receptors. A member of the CALCOCO family, CALCOCO1 is forms a calphoglin complex with PPA1 and PGM 1 and contains multiple functional domains through which it acts as a component of both the androgen signaling pathway and the Wnt/β-catenin signaling pathway. CALCOCO1 exists as three alternatively spliced isoforms (termed Q9P1Z2-1, 2 and 3), which are encoded by genes mapping to human chromosome 12q13.13 and mouse chromosome 15 F3.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human tonsil
Recommended dilution: 40-200 Predicted cell location: Cytoplasm or Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 40-200
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml225479(CALCOCO1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml225479(CALCOCO1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:77 kDa
Positive control:231, Jurkat, Hela, 293T cell and Mouse heart tissue lysates
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-5: 231, Jurkat, Hela, 293T cell and Mouse heart tissue lysates
Primary antibody: ml225479(CALCOCO1 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 seconds
ELISA
Recommended dilution: 5000-10000
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Anti-CALCOCO1 antibody
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