Product overview
Description Anti-SPON1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human SPON1
Reactivity Human, Mouse, Rat
Content 0.5 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol SPON1
Full name spondin 1, extracellular matrix protein
Synonyms f-spondin; VSGP/F-spondin
Swissprot Q9HCB6
Target Background
F-Spondin, also designated Spondin-1 or vascular smooth muscle growth-promoting factor, is a member of the subgroup of the Thrombospondin type 1 class molecules. F-Spondin is a secreted, extracellular matrix-attached protein which patterns axonal trajectories by promoting adhesion and outgrowth of commissural axons, in addition to inhibiting outgrowth of motor axons.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml262300(SPON1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:91 kDa
Positive control:Mouse brain and lung tissue
Recommended dilution: 200-1000