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- sigma
- 14610C
- 美国
- 2025年07月19日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
sigma
- 保存条件：
2-8°C
- 规格：
500ML/1000ML
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文献和实验that were previously immunized and then boosted IV 72 hours prior to hybridization. Media: Auto-Pow (powdered MEM from Flow Labs) with sodium bicarbonate plus non-essential amino acids, penicillin-streptomycin, L-glutamine and HT. For the serum-containing media
at least twice. If convinced that the subclone is monoclonal, checking the isotype is possible . If not convinced that the hybrydoma is monoclonal, reclone a second time. Cloning of Hybridoma Make an accurate cell count of cells
of 20% solution to each litre media. SYNTHETIC MEDIA (selective media) - 0.67% yeast nitrogen base without amino acids (Difco) 0919 15 3 - 1.5% agar (if needed for plates). Also add 1 pellet
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