Yeast Media

 

YEPD (non-selection)

-	1% yeast extract
-	2% peptone
-	1.5% agar (if needed for plates)

After autoclaving add glucose to 2% by adding 100 ml of 20% solution to each litre media.

SYNTHETIC MEDIA (selective media)

-	0.67% yeast nitrogen base without amino acids (Difco) 0919 15 3
-	1.5% agar (if needed for plates). Also add 1 pellet of NaOH per litre of agar to avoid mushy plates.

After autoclaving add 100 ml of 20% glucose (or galactose) solution and 100 ml of 10X Dropout solution to each litre media.

10X DROPOUT SOLUTION

-	L-Isoleucine 300 mg/L
-	L-Valine 1500mg/L
-	L-Adenine hemisulfate salt 200 mg/L
-	L-Arginine HCl 200 mg/L
-	L-Lysine HCl 300 mg/L
-	L-Methionine 200 mg/L
-	L-Phenylalanine 500 mg/L
-	L-Threonine 2000mg/L
-	L-Tyrosine 300 mg/L
-	L-Uracil 200 mg/L
-	L-Histidine HCl monohydrate 200 mg/undefined Sigma H9511
-	L-Leucine 1000mg/undefined Sigma L1512
-	L-Tryptophan 200 mg/undefined Sigma T0271

~undefined    The 10X Dropout solution does not contain Histidine, Leucine and Tryptophan. These components are used to make selective media to select for yeast transformants.

~undefined_A_better_option_is_to_purchase_selective_dropout_amino_acid_mix._CSM_~FHis~E~FLeu~E~FTrp_~F~F_BIO_101_~ACat._10427~F100~B_and_add_either_His~E_Leu_or_Trp_as_needed.~K~Hp~M~2~1~Kp~M~2~1~0