- 询价
- gelatins
- JLC224087
- 上海
- 2025年07月15日
- Rabbit
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-WDFY3 antibody
- 宿主：
Rabbit
- 浓度：
1.1 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-WDFY3 antibody
Cat. No. JLC224087
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-WDFY3 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human WDFY3
Reactivity Human, Mouse
Content 1.1 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol WDFY3
Full name WD repeat and FYVE domain containing 3
Synonyms ALFY; ZFYVE25
Swissprot Q8IZQ1
Target Background
This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Nucleus
Positive control: Human liver cancer
Recommended dilution: 50-300 Predicted cell location: Cytoplasm and Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml224087(WDFY3 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml224087(WDFY3 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:395 kDa
Positive control:Hela cell lysate
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: Hela cell lysate
Primary antibody: ml224087(WDFY3 Antibody) at dilution 1/650
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 second
ELISA
Recommended dilution: 5000-10000
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
