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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-ATG9A antibody
- 宿主:
Rabbit
- 浓度:
0.2 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-ATG9A antibody
Cat. No. JLC221877
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ATG9A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ATG9A
Reactivity Human, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ATG9A
Full name autophagy related 9A
Synonyms mATG9; APG9L1; MGD3208
Swissprot Q7Z3C6
Target Background
Autophagy-related protein 9A is a protein that in humans is encoded by the ATG9A gene. Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml221877(ATG9A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221877(ATG9A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:94 kDa
Positive control:293T cells
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: 293T cells
Primary antibody: ml221877(ATG9A Antibody) at dilution 1/240
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 1000-2000
Cat. No. JLC221877
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ATG9A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ATG9A
Reactivity Human, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ATG9A
Full name autophagy related 9A
Synonyms mATG9; APG9L1; MGD3208
Swissprot Q7Z3C6
Target Background
Autophagy-related protein 9A is a protein that in humans is encoded by the ATG9A gene. Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml221877(ATG9A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221877(ATG9A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:94 kDa
Positive control:293T cells
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: 293T cells
Primary antibody: ml221877(ATG9A Antibody) at dilution 1/240
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 1000-2000
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Anti-ATG9A antibody
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