- 询价
- gelatins
- JLC221744
- 上海
- 2025年07月09日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-DNAJB1 antibody
- 宿主：
Rabbit
- 浓度：
0.3 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-DNAJB1 antibody
Cat. No. JLC221744
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-DNAJB1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human DNAJB1
Reactivity Human, Mouse
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol DNAJB1
Full name DnaJ (Hsp40) homolog, subfamily B, member 1
Synonyms Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Swissprot P25685
Target Background
Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Nucleus
Positive control: Human lung cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm and Nucleus
Positive control: Human gastric cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml221744(DNAJB1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml221744(DNAJB1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:38 kDa
Positive control:293T cells
Recommended dilution: 500-2000
Gel: 15%SDS-PAGE
Lysate: 40 μg
Lane: 293T cells
Primary antibody: ml221744(DNAJB1 Antibody) at dilution 1/750
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 second
ELISA
Recommended dilution: 2000-5000
Cat. No. JLC221744
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-DNAJB1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human DNAJB1
Reactivity Human, Mouse
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol DNAJB1
Full name DnaJ (Hsp40) homolog, subfamily B, member 1
Synonyms Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Swissprot P25685
Target Background
Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Nucleus
Positive control: Human lung cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm and Nucleus
Positive control: Human gastric cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml221744(DNAJB1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml221744(DNAJB1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:38 kDa
Positive control:293T cells
Recommended dilution: 500-2000
Gel: 15%SDS-PAGE
Lysate: 40 μg
Lane: 293T cells
Primary antibody: ml221744(DNAJB1 Antibody) at dilution 1/750
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 second
ELISA
Recommended dilution: 2000-5000
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Anti-DNAJB1 antibody
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